Fig. 7: ILC subsets respond to HIV through cytokine secretion and surface receptor alterations. | Nature Immunology

Fig. 7: ILC subsets respond to HIV through cytokine secretion and surface receptor alterations.

From: Spatial and functional diversity of innate lymphoid cells in the human female genital tract may contribute to antiviral responses to HIV

Fig. 7: ILC subsets respond to HIV through cytokine secretion and surface receptor alterations.The alternative text for this image may have been generated using AI.

a, Representative contour plot of CD4 expression in genital T cells, EM, END and ECT ILCs. b, Percentage of ILCs expressing CD4 for each tissue type across all patients (n = 60 tissues: EM, n = 24; END, n = 19; ECT, n = 19). c, Percentage of CD4+ ILC1s and ILC3s in each tissue (n = 60 tissues: EM, n = 24; END, n = 18; ECT, n = 19). d, Comparison of CD161 MFI between CD4+ and CD4− ILCs (n = 60 tissues). e, Representative image of a CD4+ ILC1 in the ECT. f, Representative contour plot of CD4 and CCR5 co-expression on ILCs (left) and quantification (right) (EM, n = 24; END, n = 24; ECT, n = 19). g, Histogram of IFNγ (top) and CD107a (bottom) expression in CD161+ and CD161− ILC1s. Quantification of intracellular IFNγ and CD107a in control and HIV-stimulated ILC1s (n = 9). The connecting line represents one patient. h, Contour plot showing CD4 and CD107a expression in control ILC1s and stimulated with HIV and gp120. Quantification of CD4+ ILC1s with and without HIV stimulation (right) (n = 10 tissues). i, Percentage of CD161− ILC1s that were CD4+ (left), IFNγ+ (middle) and CD107a+ before and after gp120 stimulation (n = 9 tissues) j, Histogram of IL-22 (top) and CD107a (bottom) expression in CD161+ and CD161− ILC3s. Quantification of intracellular IL-22 and CD107a in control and HIV-stimulated ILC3s (right). The connecting line represents one patient (n = 9). k, Contour plot representing CD161 and NKp44 expression on endometrial (top left) and ectocervical (bottom left) ILC3s stimulated with and without HIV. Percentage of CD161hi (top right) (n = 12 tissues) and NKp44+ (n = 6 tissues) (bottom right) ILC3s with HIV stimulation. l, Percentage of IL-22+ (top) and CD107a+ (bottom) CD161+ ILC3s with and without gp120 stimulation (n = 8 tissues). Each dot represents a biological replicate. Horizontal lines represent the median ± IQR. Statistical analysis was performed using the non-parametric Kruskal–Wallis test (two-sided) with Dunn’s post hoc test correction for multiple comparisons. Paired comparisons were done with the Wilcoxon non-parametric test (two-sided).

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