Extended Data Fig. 1: Genital helper ILCs are distinct from NK cells.
(a) Contour plot of CD335 expression in ILCs (left) and NK cells (right). (b) Contour plot of CD94 expression vs CD127 expression in lineage negative cells. (c) Contour plot of CD56 expression in lineage negative cells (left) with CD94 expression overlayed in color (middle). Quantification of CD94+ Lineage negative CD56 + CD127- cells and ILCs (right) (n = 6 tissues). (d) Quantification of percent of ILCs expressing CD56 (left). Percent of CD56 + ILC1s and ILC3s across tissues (right). EM = 22, END = 15, ECT = 16. (e) Contour plots of CD203c expression in each tissue comparing total CD45+ (top) and genital ILCs (bottom). Quantification of CD203c expression in CD45+ and ILCs (right) (n = 9 tissues). (f) Contour plot of cells expressing CD34 in total CD45- (top) and genital ILCs (bottom) (n = 6). (g) Contour plots depicting percentage of CD123 expression in total CD45+ lineage- but including CD11c+ cells (left) and CD45+ lineage- CD11c- cells (middle). Quantification of CD123 expression (right) (n = 3 tissues) (h) Representative contour plot depicting CD294 and CD4 expression in CD3+ cells (left) and ILCs (right). Percent of CD294 + T-cells (CD3+) in different donors (n = 43 tissues). (i) CD127 MFI comparison between ILC1s and ILC3s in all tissues. (n = 56 tissues). (j) Representative contour plot of IL-5 (left), IL-17 (middle), and IL-13 (right) expression in genital ILCs. (k) Contour plot of Granulysin expression in genital ILCs (left) and NK cells (right) (n = 56 tissues). Each dot represents a biological replicate. Horizontal lines represent the median ± IQR. Statistical analysis was performed using the non-parametric Kruskal–Wallis test (two-sided) with Dunn’s post-test correction for multiple comparisons. Paired comparisons were done with the Wilcoxon non-parametric test. *P < 0.05; **P < 0.01; ****P < 0.0001.
