Fig. 2: pDCs are dispensable during MCMV infection.
CTR and pDC-less mice were infected (intraperitoneally) with MCMV Smith (1 × 105 p.f.u.) and analyzed p.i. a, Cytokine concentrations were analyzed at 0 and 1.5 days p.i. Each dot represents an individual. Data (mean ± s.e.m.) are pooled from two independent experiments (n = 3 for 0, n = 7 for 1.5) and analyzed using an unpaired and nonparametric two-sided multiple t-test (Mann–Whitney) with Holm–Sidak correction method. b, Survival of CTR and pDC-less mice infected with 1.4 × 105 p.f.u. of MCMV Smith was analyzed in a Kaplan–Meier curve with a log-rank (Mantel–Cox) test (n = 10 for both strains). c, Viral titers were measured by RT–qPCR in the spleen, liver and lungs of CTR and pDC-less mice 3 days p.i. Data are pooled from two independent experiments (spleen n = 9 CTR and 7 pDC-less, liver n = 7 CTR and 6 pDC-less, lungs n = 8 for both strains). Means are indicated by a horizontal bar. An unpaired and nonparametric two-sided multiple t-test (Mann–Whitney) with Holm–Sidak correction method was used for the statistical analysis. d, Expression of indicated genes was analyzed by RT–qPCR in indicated organs of uninfected or MCMV-infected CTR, pDC-less and Ifnar1KO mice at indicated days p.i. Data were normalized to expression level of Actin gene. The data (mean + s.e.m.) shown are from two independent experiments (n = 4 for each condition and time point). A nonparametric one-way analysis of variance (ANOVA) (Kruskal–Wallis test with Dunn’s correction) was used for the statistical analysis. e, The percentages of Granzyme B (GzmB)+, IFNγ+ and CD69+ splenic NK cells (mean ± s.e.m.) were determined in CTR and pDC-less mice at indicated days p.i. (n = 4 for 0, 8 for 1.5, n = 7 for 2 and n = 11 for 3 days p.i. for both strains, except n = 8 for IFNγ staining 3 p.i.). Data were analyzed using an unpaired and nonparametric two-sided multiple t-test (Mann–Whitney) with Holm–Sidak correction.
