Fig. 6: IAV-induced lung inflammation and cytokine production are reduced in pDC-less mice.
a–d, Representative images of lung hematoxylin and eosin-stained sections of CTR (a,b) and pDC-less (c,d) mice 8 days p.i. (a,b) marked broncho-interstitial pneumonia with terminal bronchiole filled with degenerated neutrophils; (c,d) mild broncho-interstitial pneumonia. Magnification is indicated. e, Semiquantitative scoring of lung lesions according to Supplementary Table 1. The data (mean ± s.e.m.) pooled from two independent experiments (n = 9 CTR, 8 pDC-less) were analyzed using a two-tailed nonparametric Mann–Whitney U-test. f, Absolute numbers of whole and CD45+ lung cells of CTR and pDC-less mice during infection. Data (mean ± s.e.m.) are pooled from two independent experiments (n per strain = 5 at 0, 7 at 3, 8 at 6, 7 CTR and 8 pDC-less at 8 days p.i.). g, Percentages of lung myeloid and lymphoid cells during infection. n = 7 per strain, except cMo, n = 4 per strain and eosinophils, n = 14 CTR and 13 pDC-less at 0, 7 per strain at 3, 8 CTR and 7 pDC-less, except eosinophils, n = 17 CTR and 14 pDC-less, at 6, 7 CTR and 8 pDC-less, except pDCs and other dendritic cells, n = 4 per strain. Data in f,g were analyzed using an unpaired and nonparametric two-sided multiple t-test (Mann–Whitney) with Holm–Sidak correction. h, Survival of IAV-infected CTR and pDC-less mice treated with anti-Ly6G (1A8, triangle) or isotype control (IC; circle) antibodies 5 and 6 days p.i. Individuals pooled of two independent experiments were analyzed using a Kaplan–Meier survival plot with a log-rank (Mantel–Cox) test. IC, n = 8 per strain; 1A8, n = 9 per strain. i, Cytokine concentrations in the BAL of CTR and pDC-less mice. Each dot represents an individual. Data shown (mean + s.e.m.) pooled from two experiments were analyzed using an unpaired and nonparametric two-sided multiple t-test (Mann–Whitney) with Holm–Sidak correction. n = 3 CTR and 4 pDC-less at 0, 3 CTR and 2 pDC-less at 2, 7 per strain at 3, 6 per strain at 6 and 7 per strain at 8 days p.i.
