Extended Data Fig. 1: Identification of transcriptionally distinct HAPC subsets. | Nature Immunology

Extended Data Fig. 1: Identification of transcriptionally distinct HAPC subsets.

From: The differentiation and function of heterogeneous thymic dendritic cell subsets require signals provided by distinct thymocyte cell types

Extended Data Fig. 1: Identification of transcriptionally distinct HAPC subsets.

Related to Fig. 1. (a) Flow cytometric gating strategy used to sort HAPCs for scRNA-seq analysis. (b) UMAP visualization showing major HAPC populations identified from 8404 FACS-sorted HAPCs (and residual thymocytes) from adult C57BL6/J thymi- B cells (Cd19), cDCs (Zbtb46, Itgax), pDCs (Itgax, Siglech), macrophages/monocytes (Csf1r, C1qa), granulocytes (Camp), and thymocytes (Lck) from n = 2 mice. (c) Feature plots showing expression levels of select genes associated with the major HAPC subsets overlaid on the UMAP visualization from (b). (d) Heatmap displaying normalized expression of the top 10 differentially expressed genes in each transcriptionally distinct cDC cluster from Fig. 1a. Arrows highlight select genes specific to the transcriptionally distinct cDC1, cDC2, and aDC clusters. (e) Violin plot displaying normalized expression levels of Zbtb46 gene in Ly6d+ cDCs and pDCs. (f) To compare our transcriptionally distinct DC clusters to those reported in the literature, we carried out Spearman correlation analysis of thymic cDC clusters from our dataset with cDC and other clusters in the dataset from11.

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