Extended Data Fig. 6: Assessment of signalling pathway activation in CBL deficient cells. | Nature Immunology

Extended Data Fig. 6: Assessment of signalling pathway activation in CBL deficient cells.

From: Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function

Extended Data Fig. 6: Assessment of signalling pathway activation in CBL deficient cells.

(a-f) Western blots of wildtype and CBL Y371C KI REH cells upon CD38 crosslinking with daratumumab for the indicated time periods (min/h). (b,c, e,f) Shows quantifications of three biological replicates. Mean ± s.d. Statistical significance was assessed with Mann Whitney tests. *p<0.05. (g,h) ERK phosphorylation upon CD38 crosslinking in gene-edited HSPC-derived CD19+ B progenitors. Total CD19+ (g) or CD19+CD20high (h) B progenitors were sorted from co-cultures edited at the AAVS1 or CBL locus. Cells were stimulated for 15 min with daratumumab, followed by fixation, permeabilization and intracellular staining for phosphorylated ERK (pERK). n = 2 biological replicates. Bars show the mean.

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