Extended Data Fig. 6: Binding affinities and neutralization activities for 007.
a, V3 bNAb Fabs were injected over BG505 (top) or BG505-DS (bottom) SOSIP trimers, and a 1:1 binding model was used to fit the experimental data and derive kinetic constants. Experimental data are shown by colored lines and predicted values are shown by black lines. A representative SPR sensorgram of duplicate experiments is shown. b, Molar IC50 values for IgG1, IgG3, bispecific IgG1, and Fab forms of 007 against a panel of HIV-1 pseudoviruses. The data are the same as in Fig. 3b, but rearranged in order of decreasing potency (that is, increasing IC50 values) of the 007 bispecific IgG1 to highlight the positive correlation between decreasing monovalent potencies and increasing bispecific / bivalent IgG1 MNRs. c, A plot showing the positive correlation between the 007 bispecific IC50 values and the bispecific / bivalent IgG1 MNRs. The Spearman correlation coefficient (rs) and p-value (< 0.0001) were determined by a two-tailed Spearman’s rho test and are illustrated next to the plot.
