Fig. 2: 007 recognizes an N332gp120 glycan-independent V3 epitope.
a, Left: overviews of the four structural classes identified of SOSIP trimers with 0-, 1-, 2- or 3-bound 007 Fabs per trimer. Right: the number of particles used in each of the final reconstructions. b, Overlay of 007 with V3-targeting bNAbs (PDB codes 5C7K, 5T3Z, 6CH7, 4JM2). c, Alignment of 007 VH and VL to their predicted germline V gene segments. 007 residues within 4 Å of protein or glycan components on Env are indicated by colored circles. d, Structure overview, highlighting proximal glycans. e, Protein contacts between 007 and Env. f, Protein contacts between EPTC112 and Env (PDB code 8C8T). g, EM density highlighting the N156gp120 (left) and N301gp120 (right) glycans. h, Neutralizing activity (IC50) of 007 and 10-1074 against HIV-1 pseudoviruses produced in the presence of kifunensine (kif). wt, wild type. i, Comparison of glycoform abundance between total BG505 SOSIP and 007-bound SOSIP at N301gp120 determined by LC-MS/MS. Points represent the replicative measurements (three for total BG505 and two for bound BG505), and bar graphs represent the mean of replicative measurements. Differences between groups were evaluated for statistical significance based on P values calculated using Welch’s t-test (two-sided). The P values are *0.04468 (for Man-6), **0.001095 (for Man-5) and ***0.04321 (for Man-4).
