Fig. 7: LC-Mo is linked to dysregulation of CD14+ monocyte function in LCAM.
a, Volcano plot showing DGE in CD14+ monocytes from LC-Mohi (>10% of CD14+ monocytes exhibiting the LC-Mo state) versus LC-Molo (<10% of CD14+ monocytes exhibiting the LC-Mo state) LCAM (n = 7), LCAS (n = 5) and RLC (n = 6) samples collected from cohort 1 at month 1.7–2.9 and month 6–8.9 time points and stimulated with P. aeruginosa for 4 h. Selected significant labeled genes with an adjusted P value of <0.05 are shown in red, whereas all other genes are shown in gray. Data were analyzed by Wilcoxon rank-sum test, Benjamini–Hochberg method-adjusted P < 0.05. b, GSEA enrichment of pathways in nonstimulated LC-Mohi versus LC-Molo and stimulated LC-Mohi versus LC-Molo (unstimulated LC-Mohi, n = 11; unstimulated LC-Molo, n = 23; stimulated LC-Mohi, n = 4; stimulated LC-Molo, n = 14). Pathways plotted with an adjusted P value of <0.1 (Kolmogorov–Smirnov-based test with permutation-derived P values, adjusted using the Benjamini–Hochberg method). c, Dot plot showing gene expression of chemokine and cytokine genes (top) and IFN response genes (bottom) in stimulated and nonstimulated CD14+ monocytes from LC-Mohi and LC-Molo as in a.
