Fig. 7: CD38 is essential for persistence of antigen-specific T_reg cells in the inflamed CNS.

EAE was induced in wild-type C57BL/6 mice. a,b, Percentages of MOG–I-Ab tetramer-binding T_conv and T_reg cells in the CNS at different stages of EAE. c, CD38 expression in MOG–I-Ab⁻ versus MOG–I-Ab⁺ T_conv and T_reg cells across EAE stages. Each symbol in b and c represents an individual mouse. Data are shown as mean + s.d. P values were calculated using a two-way ANOVA with a Tukey’s HSD test (n = 5 biological replicates). d, Mixed BM from Cd38^+/+ (CD45.1) and Cd38^−/− (CD45.2) mice was transferred into sublethally irradiated Rag1^−/− recipients. EAE was induced in BM-reconstituted mice, and CNS cells were collected 20 days after MOG_35–55 immunization. Percentages of MOG–I-Ab-binding Cd38^+/+ CD45.1⁺ and Cd38^−/− CD45.2⁺ T_conv (TCRβ⁺CD4⁺Foxp3⁻; e) and T_reg cells (TCRβ⁺CD4⁺Foxp3⁺; f). Each symbol in e and f represents an individual mouse. Data are shown as mean + s.d. The P value was calculated using a paired two-tailed Student’s t-test (n = 6 biological replicates).

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