Extended Data Fig. 1: Stress-tolerant Treg cells populate distinct niches in the chronically inflamed CNS. | Nature Immunology

Extended Data Fig. 1: Stress-tolerant Treg cells populate distinct niches in the chronically inflamed CNS.

From: CD38 endows local antigen-specific Treg cells with stress resilience for control of compartmentalized CNS inflammation

Extended Data Fig. 1: Stress-tolerant Treg cells populate distinct niches in the chronically inflamed CNS.

EAE was induced in Foxp3 (GFP) mice, and on day 45 after MOG35-55 immunization, CNS tissue was collected, and serial sections of the brain were analyzed for the presence of CD4+Foxp3+ T cells (a). Representative coronal section, scale bar, overview 500 μm; scale bars, high power magnifications 50 μm. (b, c) Absolute number of Treg cells per coronal section in relation to the distance from the bregma, around the third ventricle (b) or the meningeal space (c). (d) Visualization of the Treg cell hotspot in the third ventricle area in coronal mouse brain atlas schemes (according to Paxinos and Franklin, The mouse brain in stereotaxic coordinates). (e) Quantification of Foxp3+ Treg cells as a fraction of all CD4+ T cells in the third ventricle area and the meningeal space. (b, c, e) Sample size: n = 3 biological replicates.

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