Fig. 1: scRNA-seq of pulmonary melanoma identifies gene signatures in CD206^hi IMs, CD206^lo IMs and recMacs.

a, Unbiased UMAP clustering of scRNA-seq data from flow-sorted extravascular CD64⁺CD11b⁺ mononuclear phagocytes isolated from the lungs of C57BL/6 WT mice on day 16 after intravenous injection of 4 × 10⁵ B16F10 melanoma cells. Clusters include AMs, IMs, recruited macrophages (recMacs), dendritic cells 1 (DC1), dendritic cells 2 (DC2), inflammatory DCs (Inf DC2), migratory DCs (Mig DCs), pre-DCs, plasmacytoid DCs (pDCs), neutrophils, eosinophils, basophils, mast cells, CD8⁺ T cells, CD4⁺ T cells, regulatory T (T_reg) cells, double-negative T (DN T) cells, γδ T cells, natural killer (NK) cells, NK progenitors, B cells, innate lymphoid cells (ILC2), melanoma cells and cycling cells. b, Key signature genes defining the unbiased clusters as in a. c, UMAP of Pf4⁺C5ar1⁺Mafb⁺ IMs and Ly6c2⁺Fn1⁺Vcan⁺ recMacs as in a. d, Feature plots showing the expression of C1qb and Pf4 in IMs (left), expression of Folr2, Cd163, Mmp9 and Lyve1 in CD206^hi IMs (middle) and expression of Ccr2, Mmp12, Mmp13 and Tmem119 in CD206^lo IMs (right). e, Feature plots showing expression for Fn1, Ly6c2, Vcan and Thbs1 in recMacs. f, Feature plots showing the expression of Cxcl13, Cxcl9 and Cxcl10 in CD206^hi IMs (left) and Spp1, Vegfa, Arg1 and Cd274 in recMacs and Trem2 in recMacs and IMs (right).

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