Extended Data Fig. 5: Spatial transcriptomic visualization of Cxcl13 expression in CD206hi IMs in lung adenocarcinoma and melanoma.
From: Chemokine-defined macrophage niches establish spatial organization of tumor immunity
(a) Spatial RNA expression of Cd163, Folr2, and Cxcl13 in lungs of WT C57BL/6 mice 16 days after intravenous injection of 4×105 KPAR1.3 adenocarcinoma (n = 2) and B16F10 melanoma cells (n = 2). Scale bar, 1000 µm. (b) High-resolution imaging of spatial transcriptomics (as in a) showing that Folr2⁺ CD163⁺ (purple and blue) CD206hi IMs coexpress Cxcl13 (orange), located close to Epcam⁺ (green) bronchial airways. Scale bar, 100 µm (c) High-resolution imaging of one adenocarcinoma (as in a) spatial expression analysis showing Folr2⁺ (blue) CD206hi IMs lining the bronchial airways coexpress Cxcl13 (orange), while Epcam⁺ (green) bronchial epithelial cells and Cd3⁺ T cells (pink) do not. Note: Other cell types in the lungs besides CD206hi IMs also express Cxcl13 (as in a). Scale bar, 50 µm.
