Fig. 3: Compartmentalized IM and recMac populations organize chemokine landscapes in lung adenocarcinoma.
From: Chemokine-defined macrophage niches establish spatial organization of tumor immunity
a, Xenium spatial transcriptomic analysis showing in situ gene expression of markers of structural cells (Acta2, Tubb3, Lyve1, Pecam1 and Epcam), AMs (Car4 and Chil3), recMacs (Fn1, Vcan and Plac8), IMs (Mafb, C1qc, Mmp12, Mmp9 and Cd163) and chemokines (Cxcl9, Cxcl13, Cxcl14 and Cxcl16) in the lungs of C57BL/6 WT mice at day 16 post-intravenous injection of 4 × 105 KPAR1.3 adenocarcinoma cells. White arrows, activated stromal structures within the tumor microenvironment. Scale bar, 1,000 µm. b, Representative H&E-stained section of the lung of C57BL/6 WT mice at day 16 post-intravenous injection of 4 × 105 KPAR1.3 adenocarcinoma cells, as in a. Scale bar, 1,000 µm. c, High-resolution imaging of spatial transcriptomics as in a showing expression of Folr2, CD163, Cxcl13 and Epcam. Red arrows highlight Cxcl13+CD206hi IMs. Scale bar, 100 µm. d,e, Spatial transcriptomics quantification of tumor associated macrophages in adenocarcinoma (n = 2) (d) or melanoma (n = 2) (e).
