Extended Data Fig. 2: A spontaneous genetic model of lung adenocarcinoma.
From: Chemokine-defined macrophage niches establish spatial organization of tumor immunity
Agertm2.1(Cre/ERT2)Blh/2 J mice were crossed with B6.129-Krastm4Tyj Trp53tm1Brn/J (KP; LSL-K-ras G12D; p53LoxP mice). Ager(Cre/ERT2)+/−Kras+/−Trp53+/− were selected for BM chimera recipients. Mice were divided into two groups and reconstituted with BM from either Cx3cr1DTR control or Pf4CreCx3cr1DTR mice. Six weeks after BM transplant and immune cell reconstitution, chimeric mice were fed tamoxifen chow for 30 days to activate AgerCre allele. After 30 days on tamoxifen chow, mice were returned to regular chow and received intravenous injections of diphtheria toxin (DT; 500 ng per mouse) once weekly for 14 weeks, after which mice were harvested for tumor analysis.
