Extended Data Fig. 3: Spatial transcriptomics reveals abundant recMacs and IMs with distinct chemokine programs in lung adenocarcinoma.
From: Chemokine-defined macrophage niches establish spatial organization of tumor immunity
(a) Xenium spatial expression analysis of the lungs of two naïve WT C57BL/6 mice showing the expression of Acta2 (alpha-smooth muscle actin) (b) Spatial expression analysis of the lungs of WT C57BL/6 mice 16 days after intravenous injection of 4×105 KPAR1.3 adenocarcinoma cells shows various genes in different compartments including: nerves (Nes), lymphatic vessels (Pdpn), alveolar macrophages (Ear1), monocyte/macrophage markers (Cd14), recruited macrophages (Clec4n, Cd9), CD206hi IMs (Folr2), B cells (Cd19), T cells (CD3e), chemokines (Ccl3, Ccl4, Ccl6, Ccl7, Ccl8, Ccl9, Ccl17, Ccl22, Cxcl10, Cxcl3). Macrophages (Mafb) and dendritic cells (Zbtb46, Flt3, Xcr1). Scale bar, 1000 µm.
