Extended Data Fig. 9: Eomes ablation alters CD4⁺ T cell differentiation in vivo. | Nature Immunology

Extended Data Fig. 9: Eomes ablation alters CD4⁺ T cell differentiation in vivo.

From: A unique CD4⁺ T cell subset expressing granzyme K is regulated by transcription factor EOMES and important for T cell-mediated intestinal inflammation

Extended Data Fig. 9: Eomes ablation alters CD4⁺ T cell differentiation in vivo.

a–m, Flow cytometric analysis of transcription factors and effector molecules in donor-derived CD4⁺ T cells from colonic lamina propria (LP) and mesenteric lymph nodes (mLN) (n = 5 or 6). Representative plots showing EOMES and Gzmk-tdTomato expression, with quantification of the frequency of Gzmk-tdTomato⁺ cells in mLN (a). Representative plots (left) and frequencies (right) of perforin⁺ (b) and GZMB⁺ (c) cells in mLN. d–m, Frequencies of T-bet⁺ (d,e), IFNγ⁺ (f,g), RORγt⁺ (h,i), IL-17A⁺ (j,k), and FOXP3⁺ (l,m) cells in LP (upper), mLN (lower). n, Frequencies of CD8α⁺ cells in CD4⁺ T cells in LP. o,p, Disease severity in Rag1−/− recipients of naive CD4⁺ T cells from Gzmk−/− (n = 5) or control (n = 5) donors. Body weight change (o); colon length at endpoint (p). Data are shown as mean ± s.e.m. and are representative of three independent experiments. Each data point represents an individual mouse. Exact P values are shown in each graph. Statistical significance was assessed by two-tailed unpaired t-test (a–n,p) and two-way ANOVA with Bonferroni’s post hoc test (o).

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