Extended Data Fig. 4: CTH binds to Pam3CSK4 and bacterial lipoprotein.
From: Cytosolic CTH senses bacterial lipoproteins and drives noncanonical inflammasome activation
a, Coomassie blue staining of recombinant CTH proteins purified from HEK293F cells. b, Pulldown assay of BMDM lysates from wild-type mice with biotin-Pam3CSK4 or biotin-GFP (control) using streptavidin beads and immunoblotted for CTH. c, Pulldown assay of BMDM lysates from wild-type mice with his-tagged Lpp purified from S. aureus or HEK293F cells. NLRP3 fragment (aa 600–920) as a control. Data are representative of three independent experiments with similar results in a-c.
