Extended Data Fig. 8: CTH is beneficial for the host to resist bacterial infections.
From: Cytosolic CTH senses bacterial lipoproteins and drives noncanonical inflammasome activation
a, Kaplan–Meier survival curve of wild-type, Lyz2CreCthflox/flox (CTH cKO), Casp11−/− and Casp1−/− mice challenged intraperitoneally with poly(I:C) (2 mg/kg) for 6 h, then injected intraperitoneally with 2 ×106 CFU Listeria monocytogenes (n = 6 mice per group). b, Quantification of bacterial loads in spleen, liver and kidney of mice treated as in a. Bacterial loads were measured at 72 h post-infection (n = 5 mice per group). Casp1−/− mice were excluded due to complete mortality by day 3. ND, not detected. c, ELISA assay of serum levels of IL-1β in mice treated as in a. Serum levels of IL-1β determined at 24 h post infection (n = 5 mice per group). d, Kaplan–Meier survival curve of wild-type mice challenged intraperitoneally with poly(I:C) (2 mg/kg) for 6 h, then injected intraperitoneally with 5 × 108 CFU of S. aureus with or without PAG (50 mg/kg/day) (n = 8 mice per group). e, Quantification of bacterial loads in spleen, liver and kidney of wild-type mice treated as in d. Bacterial loads were measured at 72 h post-infection (n = 5 mice per group). f, ELISA assay of serum levels of IL-1β in wild-type mice treated as in d. Serum levels of IL-1β determined at 24 h post infection (n = 5 mice per group). In a and d, Gehan-Breslow-Wilcoxon test was used for survival analysis, n = 6 per group for a and n = 8 per group for d. In b, c, e and f, data are pooled from three independent experiments (n = 5) and shown as means ± SD. Unpaired two-tailed Student’s t-test was used. Exact P values are indicated in the figures. Data are representative of three independent experiments in a-f.
