Extended Data Fig. 8: THZ1 and actinomycin D reduce expression of T (brachyury) in a concentration- and time-dependent fashion.
From: Small-molecule targeting of brachyury transcription factor addiction in chordoma
a, Immunoblot analysis of UM-Chor1 cells treated with indicated concentrations of THZ1 or DMSO for 12, 24, 36 or 48 h. The experiment was performed once. b, UM-Chor1 cells were treated with indicated concentrations of compound or DMSO for 4, 8 or 12 h and subjected to RT–qPCR. Data are expressed as the log2 fold change of transcript levels relative to vehicle-treated cells, normalized to GAPDH levels, and represent the mean ± s.d. (n = 3 biological samples measured in parallel). Results of statistical analyses of RT–qPCR data, derived from a one-sided Welch’s t-test, are reported in Supplementary Table 9. c, Response of chordoma cells to treatment with actinomycin D. Four chordoma cell lines were treated with indicated concentrations of compound and assayed for cell viability after 6 d with CellTiter-Glo. Response data are represented by a fitted curve to the mean fractional viability at each concentration relative to vehicle-treated cells; error bars represent the s.e.m. (n = 4 biological samples measured in parallel).
