Extended Data Fig. 9: Expression of ATP6V1B2, SAE1, SOX9 and TPX2 is downregulated following sgRNA-mediated T (brachyury) repression.
From: Small-molecule targeting of brachyury transcription factor addiction in chordoma
a, UM-Chor1 cells were transduced with sgRNAs targeting T or a non-targeting sgRNA control and subjected to RT–qPCR. Data are expressed as the fold-change of transcript levels relative to sgRNA control-treated cells, normalized to GAPDH levels and represent the mean (n = 2 biological samples measured in parallel, represented by black points). * P < 0.05; ** P < 0.01; *** P < 0.001; P values were derived from a one-sided Welch’s t-test. Exact P values and effect sizes are reported in Supplementary Table 9. b, Immunoblot analysis of UM-Chor1 cells transduced with sgRNAs targeting T or a non-targeting sgRNA control. SgRNA treatment was performed once and immunoblotting was performed twice (one representative experiment displayed).
