Extended Data Fig. 5: Transcriptome analysis by RNA-seq and identification of miR-140-5p-G targets by TargetScan.
From: Gain-of-function mutation of microRNA-140 in human skeletal dysplasia
a, Expression levels of genes within the neighborhood of the miR-140 gene locus. Results represent mean ± s.e.m. (n = 3 animals). b, Workflow of target prediction by TargetScan v7.0. c,d, Venn diagrams showing overlaps of genes with conserved sites (c) and genes with conserved and non-conserved sites (d) for miR-140-5p-WT, -5p-G, -3p.1, and -3p.2. e, Conservation frequency of target sites for miR-140-5p-WT, -5p-G, -3p.1, and -3p.2. The target sites of miR-140-5p-G are less conserved than those of WT miR-140 species. Classification of miRNAs (broadly conserved, conserved, poorly conserved, and others) is based on the TargetScan information. Center lines show medians; box limits indicate the 25th and 75th percentiles; whiskers extend to 1.5× the interquartile range.
