Extended Data Fig. 8: Analysis of CAG repeat content in the human and mouse genomes.
From: Allele-selective transcriptional repression of mutant HTT for the treatment of Huntington’s disease
a–d, Distribution of all ≥6× CAG arrays with 0 (a,c) or up to three (b,d) mismatches per CAG hexamer subsite to capture longer, discontiguous repeat tracts (see Methods and Supplementary Tables 8–15) in the human (hg38, orange) and mouse (mm10, blue) genomes. a,b, All CAG arrays represented. c,d, CAG arrays located within 1 kb of a TSS. e,f, Ortholog mapping and corresponding CAG counts for the top 100 genes with the largest CAG repeats within 1 kb of a TSS in the mouse (e) or human (f) genome. A line connects the total CAG count for the mouse gene and its human ortholog (e), or the human gene and its mouse ortholog (f). Search criteria were ≥6× CAG repeats within 1 kb of the TSS with zero (left panel) or up to three (right panel) mismatches per 6× CAG subsite. The number of genes with either no CAG array in the orthologous gene or no ortholog in the other genome is shown at the bottom right of each panel. For clarity, only the top 100 genes are shown; lists were sorted on CAG length, then absolute distance from the TSS. Overlaps may be present for some entries due to multiple ortholog relationships sharing the same CAG counts. See Supplementary Tables 16–19 for complete ortholog lists. g, As in (f) except that only the 12 genes that were selected for monitoring by RT–qPCR are shown (see Extended Data Fig. 9). Only genes with a human ortholog are included.
