Extended Data Fig. 2: Statistically significant differences in the gut microbiome of Lmna^G609G/G609G versus wild-type (WT) mice at different ages.

a, Total relative abundance of prevalent microbiota at the class level in the five groups studied: 1-month-old WT (n = 6), 1-month-old Lmna^G609G/G609G (n = 5), 4-month-old WT (n = 8), 4-month-old Lmna^G609G/G609G (n = 9) and 22-month-old WT (n = 8), showing data for individual mice. The low-abundance bacteria group includes all bacterial classes with less than 0.5% of total abundance. b, Results of LEfSe analysis showing bacteria that were significantly different in abundance in progeroid (blue, n = 5) versus WT (violet, n = 6) mice at 1 month of age. c, Results of LEfSe analysis showing bacteria that were significantly different in abundance between WT mice at 1 (violet, n = 6) and 4 (grey, n = 8) months of age. d, Results of LEfSe analysis showing bacteria that were significantly different in abundance between progeroid mice at 1 (blue, n = 5) and 4 (red, n = 9) months of age. e, Results of LEfSe analysis showing bacteria that were significantly different in abundance between WT mice at 4 (grey, n = 8) and 22 (green, n = 8) months of age. f, Results of LEfSe analysis of PICRUSt-HUMAnN2, showing the top five KEGG pathways (based on P value) significantly upregulated in WT and G609G at 4 months (Supplementary Table 3). p, phylum; c, class; o, order; f, family; g, genus. For a–f, analyses were performed with WT 1mo, n = 6; G609G 1mo, n = 5; WT 4mo, n = 8; G609G 4mo, n = 9; WT 22mo, n = 8.