Extended Data Fig. 8: GS-CA1 is highly sequestered in mouse but not in human serum.
a, Effect of including 50% serum (mouse or human) in the cell culture medium on the antiviral potency of GS-CA1, RPV and RAL. Center line and error bars represent mean ± s.d. EC50 fold-change values were obtained with or without the indicated serum. Data were obtained from three independent experiments, each performed with three replicate cell cultures per condition. Brackets and numbers highlight potency shift differences between the two sera. Significant P values by unpaired two-tailed Student’s t tests with Welch’s correction are indicated with an asterisk. b, Plasma drug concentrations over time in male C57Bl/6 mice (n = 3 per time point) following seven consecutive daily subcutaneous administrations of either 15 mg per kg (body weight) GS-CA1 or 160 mg per kg (body weight) LA-RPV. Symbols represent mean ± s.d. mouse serum protein binding-adjusted EC95 values for each compound, with mean fold mouse paEC95 values indicated in boxes according to day 14 drug levels.
