Extended Data Fig. 8: ASO-A (Rictor-ASO) reduces mTORC2 (but not mTORC1) activity in cortical neurons in culture.
(a-b) Cortical neurons were treated for 72 hours with different ASOs (10 μM) designed to target Rictor mRNA. Representative western blots (a) and quantification (b) show that ASO-A strongly reduces both Rictor protein level (n = 4 per group, t6 = 10.94, P = 0.0001) and mTORC2 activity (t6 = 7.25, P = 0.0010), but had no effect on mTORC1 activity (t6 = 0.33, P = 0.9846). Data are mean ± SEM. Values were compared relative to the vehicle treated group and statistics were based on two-sided t-test comparisons. n.s., not significant.
