Extended Data Fig. 2: Genetic inhibition of mTORC1 (but not mTORC2) reverses the increased brain/body weight ratio in Pten fb-KO mice. | Nature Medicine

Extended Data Fig. 2: Genetic inhibition of mTORC1 (but not mTORC2) reverses the increased brain/body weight ratio in Pten fb-KO mice.

From: Therapeutic inhibition of mTORC2 rescues the behavioral and neurophysiological abnormalities associated with Pten-deficiency

Extended Data Fig. 2: Genetic inhibition of mTORC1 (but not mTORC2) reverses the increased brain/body weight ratio in Pten fb-KO mice.

(a) Crystal violet staining of mid-sagittal brain sections from control, Pten fb-KO mice, Pten-Rptor fb-DKO and Pten-Rictor fb-DKO mice (3 mice per group were used for the experiment). (b) Body weight of control (n = 11), Pten fb-KO (n = 9), Pten-Rictor fb-DKO mice (n = 13) and Pten-Rptor fb-DKO (n = 9) was similar (F3,38 = 0.93; P = 0.443). (c) Brain weight relative to body weight in control (n = 11, 0.94 ± 0.04%), Pten fb-KO (n = 9, 2.45 ± 0.34%, vs. control, t = 5.13, P < 0.0001), Pten-Rptor fb-DKO (n = 8, 2.12 ± 0.08%, vs. Pten fb-KO, t = 3.145, P = 0.0034) and Pten-Rictor fb-DKO mice (n = 11, 2.52 ± 0.07%, vs. Pten fb-KO, t = 0.21, P = 0.7810). Box graphs are presented as median, 25th percentile, 75th percentile, minimum, and maximum of the group. Statistics were based on one-way ANOVA followed by uncorrected two-sided Fisher’s LSD method for pairwise comparisons or paired t-test. n.s., not significant.

Back to article page