Extended Data Fig. 10: Yeast-displayed affinity matured eCNTFR does not bind to CNTF but retains high affinity binding to mCLCF1. | Nature Medicine

Extended Data Fig. 10: Yeast-displayed affinity matured eCNTFR does not bind to CNTF but retains high affinity binding to mCLCF1.

From: Antitumor activity of an engineered decoy receptor targeting CLCF1–CNTFR signaling in lung adenocarcinoma

Extended Data Fig. 10: Yeast-displayed affinity matured eCNTFR does not bind to CNTF but retains high affinity binding to mCLCF1.

(a) Binding curves representing yeast-displayed wild-type CNTFR (wtCNTFR) and engineered CNTFR (eCNTFR) binding to CNTF measured by flow cytometry (n = 3 independent experiments). Data represented as mean ± S.D. (b) Binding curves representing yeast-displayed wtCNTFR and eCNTFR binding to mCLCF1 measured by flow cytometry. While eCNTFR still binds to mCLCF1 with comparable affinity to hCLCF1, there was no detectable binding to CNTF (n = 3 independent experiments). Data represented as mean ± S.D. (c) Representative cropped western blot of two independent experiments showing A549 cells treated with mCLCF1 and phosphorylation of STAT3 (Y705) in a time-dependent manner.

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