Extended Data Fig. 5: Structural and proteomic abnormalities in NEK10-deficient airway epithelium.
From: A human ciliopathy reveals essential functions for NEK10 in airway mucociliary clearance
a, SEMs of mature ALI cultures edited with the indicated sgRNAs, representative of two independent ALI differentiations. Scale bars, 100 μm (upper panels) and 1 μm (lower panels). b, Immunoblotting against the indicated proteins from lysates generated from purified cilia (lanes 2 and 4) or remaining de-ciliated mature ALI cultures (lanes 1 and 3), representative of two experiments. c, Cumulative distribution of phosphopeptides by log2[fold change] between indicated conditions. The solid (sgNEK10b) and dashed (sgNEK10c) red lines illustrate the population of depleted phosphopeptides upon NEK10 deletion. d, Table of GO classes enriched among genes (n = 395) whose peptides are depleted >1.5 fold log2[fold change] after targeting with sgNEK10b. The enrichment levels, P values, and false discovery rates are indicated. e, Cumulative distribution of phosphopeptides by log2[fold change]. Previously validated PCD proteins are in red and all other detected proteins are in black, as in Fig. 4e. f, Cumulative distribution of phosphopeptides by log2[fold change]. Previously validated non-PCD ciliopathy proteins are in red and all other detected proteins are in black, as in Fig. 4e.
