Extended Data Fig. 6: Example of ADNP gating strategy. | Nature Medicine

Extended Data Fig. 6: Example of ADNP gating strategy.

From: Phase 1/2 trial of SARS-CoV-2 vaccine ChAdOx1 nCoV-19 with a booster dose induces multifunctional antibody responses

Extended Data Fig. 6

First neutrophils are gated based on forward and side scatter. Doublets are excluded in the following two steps. Furthermore, T cells and monocytes are excluded using a double-negative gate for CD3 and CD14. The final neutrophil gate is based on CD66-positivity, after which bead+ cells are gated. Phagocytic score is calculated by multiplying the percentage of bead-positive cells with the mean fluorescence intensity (MFI) of the beads within these cells to account for the number of beads per cell.

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