Extended Data Fig. 1: Alpha diversity linked with personal factors, habitual diet, fasting, and postprandial markers.

a, Microbiome alpha diversity computed using the Shannon index correlated markers from the four categories: personal, habitual diet, fasting, and post-prandial. Reported are the five strongest positive and negative Spearman correlations for each category with p < 0.05. All correlations and p-values available in the Supplementary Table 1. b, Inter-sample microbiome distances (beta-diversity) were substantially lower, that is closer, among samples from the same individuals (two weeks apart) compared to those amongst different individuals. Gut microbial communities in monozygotic twins were slightly more similar than in dizygotic twins (Mann–Whitney U test two-sided p = 0.06), which, in turn, were more similar than unrelated individuals (p < 1e-12), even after adjusting for age (p < 1e-12). c, After excluding twin status (that is non-twin, vs. mono vs. dizygotic twins) from the model, personal factors still accounted for the greatest proportion of variance explained in overall microbial diversity, followed by dietary habits, fasting and postprandial cardiometabolic blood markers (by cumulative stepwise dbRDA). d, Cumulative (left bars) contributions and individual (right bars) contributions for each metadata variable based on Bray-Curtis dissimilarity. Box plots show first and third quartiles (boxes) and the median (middle line), whiskers extends up-to 1.5× the interquartile range.