Extended Data Fig. 3: Cellular composition and fraction of ACE2⁺TMPRSS2⁺ cells across the aggregated lung dataset.

a, Boxplot of normalized donor fractions of ACE2⁺TMPRSS2⁺ (double positive - DP) cells per cell type. The box indicates the median and first and third quartile, whiskers extend to points within 1.5 times the interquartile range. For each cell type, only donors that have at least 100 cells of the cell type were included. Cell types with at least 10 ACE2⁺TMPRSS2⁺ cells in the entire dataset were labeled, the remaining cell types were grouped under ‘Other’. Cell type labels preceded by a ‘2’ consist of cells that had no annotation available at level 3 and therefore kept their level 2 annotation. Cells with only level 1 annotations were grouped under ‘Other’. (2_Airway epithelium: n=6, 2_Olfactory epithelium: n=3, 2_fetal airway progenitors: n=5, AT1: n=60, AT2: n=92, Basal: n=56, Multiciliated lineage: n=88, Secretory: n=79, Submucosal Secretory: n=35, Other: n=180 donors.). b, Percentage of ACE2⁺TMPRSS2⁺ cells across 377 samples and with sample composition. Top: Percentage ACE2⁺TMPRSS2⁺ cells in each sample, categorized by level 3 annotations. Bottom: Sample compositions. Samples are ordered by age, with 31-week pre-term births and 39-week full-term births both set to age 0. c, Zoom in on fetal and pediatric samples of plot (b). Samples are ordered and labeled by age. Fetal samples are partitioned into first and second trimester (TM) and pediatric samples are divided into 31-week pre-term births, 39-week full term births, 3 month, 3 year, and 10 year old children. AT1, 2: alveolar type 1, 2. AT2 progenitor cells were grouped under AT2.