Extended Data Fig. 9: SGLV infection in different cells.
From: Identification of a new orthonairovirus associated with human febrile illness in China
SGLV could grow in SMMC-7721, BHK-21, and Vero cells. Images are shown 4 days after infection and the uninfected cells were used as control. SGLV infection in different cells were tested by indirect immunofluorescence assay (IFA). Different cell lines were inoculated onto 24-well plates containing cell slides. After incubation for 12 h, the cells were infected with SGLV in triplicate at a MOI of 5. The infected cell slides at 12 h post-infection were collected for IFA; anti-SGLV sheep sera or human convalescent sera were used as the first antibody. Infected cells appeared green, and nuclear appeared blue due to DAPI staining. Images are representative of at least two independent experiments.
