Extended Data Fig. 3: Extrapulmonary pathology.
From: Ad26 vaccine protects against SARS-CoV-2 severe clinical disease in hamsters
a, Anti-sense SARS-CoV-2 viral RNA (vRNA) in brainstem on day 2 following challenge. b, Higher magnification showing cytoplasmic vRNA staining in neurons in the absence of inflammation and pathology. c, Anti-sense SARS-CoV-2 vRNA staining in the lamina propria of small intestinal villus on day 2 following challenge. d, Higher magnification showing cytoplasmic and nuclear vRNA staining in an individual mononuclear cell in the absence of inflammation and tissue pathology. e, Anti-sense SARS-CoV-2 vRNA staining within the myocardium and along the epicardial surface of the heart on day 4 following challenge. f, Higher magnification showing staining of inflammatory mononuclear cell infiltrates consistent with focal myocarditis. g, Pulmonary vessel showing endothelialitis day 4 (d4) following challenge. h, Pulmonary vessel showing CD3+ T lymphocyte staining by IHC adhered to endothelium and within vessel wall, d4 following challenge. i, Pulmonary vessel showing Iba-1+ staining by IHC of macrophages along endothelium and perivascularly, d4. j, Pulmonary vessel showing minimal vascular staining for SARS-CoV-N by IHC, d4. k, Heart from (e, f) showing focal lymphocytic myocarditis as confirmed by CD3+ T lymphocyte staining l, of cells by IHC, d4. Representative sections are shown. Experiments were repeated at least 3 times with similar results. Scale bars = 500 μm (a, c, e); 100 μm (b, d, f, g-l).
