Extended Data Fig. 9: Serial tumor challenge assay.
From: Anti-GD2 CAR-NKT cells in patients with relapsed or refractory neuroblastoma: an interim analysis
a, Patient GD2-CAR NKTs and NB cell line CHLA-255 were co-cultured in a 24-well plate. Five days later, cells were harvested for quantification by trypan-blue exclusion and FACS analysis. NKT cells were then re-plated at a 1:1 effector-to-target ratio with fresh tumor cells in fresh cell culture medium to start the next round with five rounds total. b, Representative flow cytometry analysis after round 4. Summary of c) tumor cell cytotoxicity and d) GD2-CAR NKT proliferation at the end of each five-day cycle. Data shown as mean ± SD from three technical replicates; P values by two-way ANOVA with Tukey’s correction for multiple comparisons (c and d).
