Extended Data Fig. 3: Lack of misassignment due to cross reactivity in the ELISAs.
From: Seasonal coronavirus protective immunity is short-lasting
The V1 and V2 serum samples were tested in the ELISAs for each virus. The OD value V2/V1 was calculated for each infection (= fold change). For those fold changes that were above 1.40 we calculated the ratio between the fold changes for the viruses that may or may not have cross-reacted. In each panel, the ratio of the fold changes is displayed on the Y-axis. A ratio below one indicates that the fold change measured in ELISA is the highest for the virus that was found by RT-PCR in the corresponding respiratory sample. Only one patient (infected by HCoV-NL63) displayed, besides a large rise in HCoV-NL63, also a substantial rise in HCoV-229E OD, and for this patient the fold changes were similar (9.7 versus 10.3) resulting in a 1.1 ratio (far left panel, the one data-point above 1). For all other patients with ≥1.40 fold rises matched with the RT-PCR confirmed infection.
