Extended Data Fig. 7: RBM20-R636S liquid droplets reconstructed by recombinant protein expression in U2OS cells. | Nature Medicine

Extended Data Fig. 7: RBM20-R636S liquid droplets reconstructed by recombinant protein expression in U2OS cells.

From: Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs

Extended Data Fig. 7

Transfection of GFP-RBM20-R636S in U2OS cells co-imaged with DAPI and ICC for cytoskeletal elements, microtubules (α-tubulin), F-actin microfilaments (phalloidin) or intermediate filaments (vimentin), highlighting the liquid behavior of GFP-RBM20-R636S droplets “beading-up” and fusing caused by the nuclear envelope’s surface tension. Experiment repeated numerous times with identical results. Scale bars, 50 μm for all panels.

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