Extended Data Fig. 10: Workflow for FTT analysis of GFP-RBM20 transfected hPSC-CMs and model of the liquid droplet organizational logic of cardiomyocytes.
From: Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs
a, Workflow for quantitative analysis of decreased striated patterning associated with R3G accumulation in GFP-RBM20 transfected hPSC-CMs, see Methods for description of protocol. The symbol ||e|| in graph stands for ‘norm of residuals’ (square root of the sum of squares of residuals), which is a measure of ‘goodness of fit’ in regression analysis: lower numbers equal better fit. b, Infographic of RBM20 liquid pipeline for transfer of genetic information from nuclear transcription/splicing factories to sarcoplasmic Z-discs, the command center of myofibril biogenesis, across an elaborate sarcoplasmic network of cytoskeletal-linked biomolecular condensates. Conceptually, this liquid condensate pipeline organizes and manages the flow of genetic information from the cardiac genome-to-message-to-sarcomeres with RBM20 returning to the nucleus to pick-up new cargo.
