Extended Data Fig. 2: Demonstration of endocardial fibroelastosis and abnormal sarcomere organization in HMZ gene-edited pigs.
From: Dysregulated ribonucleoprotein granules promote cardiomyopathy in RBM20 gene-edited pigs
a, Gross anatomical apical segments of same hearts in (Fig. 1g) showing endocardial fibroelastosis (circled area, whitish layer) in dilated HMZ but not WT heart at postnatal day-3. Scale bar, cm. b, Study of highly focal and HMZ-specific endocardial fibroelastosis, as in circled area (Extended Data Fig. 2a) above, by Masson’s trichrome and Verhoeff-Van Gieson’s histochemical staining. Scale bar, μm. c, TEM study comparing sarcomere striation patterns in WT (upper panel) versus HMZ (middle and lower panels) 7-day pig myocardium and graph of measured Z-disc to Z-disc intervals demonstrating shorter sarcomere length and swollen Z-discs consistent with impaired myofibril biogenesis in HMZ versus WT pig myocardium. Scale bar, μm.
