Extended Data Fig. 5: Orthogonal testing for Cases S88: Klebsiella aerogenes from cerebrospinal fluid and S87: Bartonella henselae from a skin abscess.
From: Rapid pathogen detection by metagenomic next-generation sequencing of infected body fluids
a, Orthogonal confirmation of K. aerogenes by dPCR of the DNA extract. The sample was run in parallel with 9 negative controls. Out of 10 sequencing libraries, only Case S88 had positive dPCR droplets (n = 61). b, Genomic coverage of K. aerogenes from Illumina mNGS. The assembled genomic regions spanned 536,461 bp, or 9.9% of the bacterial genome. c, Orthogonal confirmation of Bartonella henselae by dPCR of the DNA extract. Positive dPCR droplets (n = 12) are seen in abscess fluid and the positive control consisting of sheared DNA from Bartonella henselae (ATCC 49882). The negative control was water.
