Extended Data Fig. 8: Radiotherapy reshapes the liver immune microenvironment.
From: Liver metastasis restrains immunotherapy efficacy via macrophage-mediated T cell elimination
a, b Flow cytometry quantification of CD8+ T-cell number (a), Ki67+, IFNγ+ and granzyme B+ CD8+ T cells (b) in the livers from mice bearing both subcutaneous tumour and liver tumour with indicated treatments. Analysed 5 days after RT. One-way ANOVA, mean ± SD, n = 6 per group. c Hepatic tissue chemokine levels in subcutaneous and liver tumour bearing mice treated in indicated fashion. Analysed 5 days after RT by Luminex; One-way ANOVA, mean ± SD, n = 5 per group. d-f ELISA analysis of culture supernatant (d, n = 3 biologically independent samples) and phenotypic analysis (e, f, n = 4 biologically independent samples) of MC38 cells 48 hours after irradiation. One-way ANOVA, **P = 0.006, ***P = 0.0003, ****P < 0.0001, mean ± SD. g Flow cytometry plot and quantification of cleaved caspase-3 in total hepatic CD8+ T cells from mice bearing both subcutaneous tumour and liver tumour, with (n = 8) or without (n = 7) liver-directed radiotherapy (RT). Unpaired two-tailed Student’s t-test, mean ± SD. h Schematic describing liver-directed radiotherapy and adoptive cell transfer. Data are representative of at least two independent experiments (a-g).
