Extended Data Fig. 6: Relationship between % edited alleles and % edited cells.
a, Representative flow cytometry plots of HSPCs simultaneously targeted at HBA1 with GFP (shown in Fig. 1c) and mPlum integration cassettes. b, Table showing % of populations targeted with GFP only, mPlum only, and both colors. Percent of edited cells was then converted to % edited alleles by the following equation: (total % targeted cells + (% dual color)*2)/2 = total % targeted alleles. c, Percent edited cells is plotted against % edited alleles for data shown in panel B. A polynomial regression (R2 = 0.9981) was used to determine an equation to convert between % edited alleles and % edited cells.
