Extended Data Fig. 1: Analysis of Cas9 sgRNAs targeting α-globin loci.

a, Table with guide RNA sequences. PAM shown in grey, and differences between HBA1 and HBA2 are highlighted in red for each guide. b, Schematic depicting locations of all five guide sequences at genomic loci. c, Representative indel spectrum of HBA1-specific sg5 generated by TIDE software.