Extended Data Fig. 10: Simvastatin does not inhibit cholesterol levels in activated T cells.
From: A T cell resilience model associated with response to immunotherapy in multiple tumor types
(a, b), Cholesterol levels upon simvastatin treatments. T cells were treated with simvastatin concurrently with anti-CD3/28 activation (a, During) or 72 hours after anti-CD3/28 activation (b, Post). The median Filipin III intensity was measured with flow cytometry at different simvastatin concentrations, with a representative plot on the left and the median intensity on the right. The gating strategy follows the same scheme in Extended Data Fig. 9c. (c, d), Simvastatin inhibits T-cell proliferation. For each condition, the T-cell expansion index was computed through CFSE staining and flow cytometry analysis. The data is shown in the same way as panels a, b.
