Extended Data Fig. 4
(A) ELISpot data (mean SFU per 106 PBMCs +/- SD for triplicate wells) are shown for whole PBMCs (black bars), CD4-depleted (CD8enr) PBMCs (navy blue bars), and CD8-depleted (CD4enr) PBMCs (orange bars) stimulated with DMSO, CD8 Pool, or 15mer pool for patients G1, G11, and G14 (pooled posttreatment timepoints). (B) Post-IVS ELISpot data for baseline and posttreatment sample PBMCs stimulated with patient-specific CD8 and minipools are shown for patients G1, G2, G3, G8, G9, G12, G13 and G4. Graphs show mean of technical duplicates (SFU per 106 PBMCs) for all except G2 CD8 Pool, where enough cells to test triplicates were available, with DMSO background subtracted. ULOQ for post-IVS assay is indicated by dotted line.
