Extended Data Fig. 7: Regulatory T cells characteristics and in vitro suppression.
From: Distinct cellular dynamics associated with response to CAR-T therapy for refractory B cell lymphoma
a, The fraction of T cells that are T-regs in samples of each timepoint, separated by CAR+ and CAR- cells. Only samples with at least 100 T cells of the relevant type are included in the analysis. A total of n = 20 baseline, n = 27 Infusion CAR-negative, n = 27 Infusion CAR-positive, n = 28 day 7 CAR-negative and n = 22 day 7 CAR-positive biologically independent samples are shown. P-values denote a two-tailed t-test without correction for multiple hypotheses. Boxes show the median, interquartile range, and maximum/minimum values. b, Average expression in IP T-regs (orange) and all other T cells (T-conv, blue) of the top 10 differentially expressed genes comparing T-regs and T-convs in IP cells. Genes classically associated with T-reg function are highlighted with arrows. c. Schematic for T-reg and CD4 control population isolation from healthy donor PBMC. d, CAR constructs used to identify CAR-Treg/CD4-CAR cells from CAR-Tconv. e,f, CFSE staining of CAR-Tconv cells co-cultured with either 25% CAR-Tregs or CD4-CAR control cells and stimulated at a 1:1 ratio with Jeko tumor targets at 72 hours. Dividing cells (red) are identified relative to unstimulated condition (blue). Each histogram represents an individual replicate, summarized in the plot on the right for all n = 3 technical replicates per construct over 1 independent experiment. P-value represents two-tailed unpaired t-test.
