Extended Data Fig. 2: PBMC cell composition differences between responders and non-responders pre- and post-infusion.
From: Distinct cellular dynamics associated with response to CAR-T therapy for refractory B cell lymphoma
a, Boxplots of the cell type frequencies for each cell type stratified by product and response. The final cell type coefficient (with its posterior 95% high density interval) and FDR value (one minus the inclusion probability) estimated by scCODA are shown. Boxes show the median, interquartile range, and maximum/minimum values. n = 20 and n = 22 biologically independent samples are shown for baseline and D7-CAR-negative samples, respectively. b, Fraction of cells which were CD8+CD4- at baseline, in IPs, and at day 7 in CAR+ and CAR- populations. Boxes show the median, interquartile range, and maximum/minimum values. n = 20 baseline, n = 30 infusion, n = 29 day 7 CAR-negative, and n = 29 day 7 CAR-positive independent samples are shown from 31 patients. c, Flow-cytometric measurements for the mean fraction of CAR positive cells which were CD8+CD4- at day 7 for n = 27 biologically independent samples. Error bars represent standard deviation. P-values represent two-tailed Mann -Whitney U tests. d, Changes in CD8+ frequencies between IP and day 7 CAR-T cells as shown in Fig. 3b, colored by CD19 status of relapse. Samples with no relapse, or relapse without a biopsy, are grayed out.
