Extended Data Fig. 7: Identified populations in healthy donors and CD19-CD28ζ CAR-transduced T cells.

a,b, Contour plots show three identified populations among T cells from a healthy donor (a) and among CD19-CD28ζ CAR-transduced T cells generated in the lab (b). Population statistics for two donors are shown as mean ± SEM on each plot. c, Percentage of FOXP3⁺CD25^High and FOXP3⁺Helios⁺ cells among CD4⁺ T cells are shown for a healthy donor (top) and for CD19-CD28ζ CAR-transduced T cells generated in the lab (bottom). Population statistics for two donors are shown as mean ± SEM on each plot. d, Cryopreserved T cells from a healthy donor were incubated with PMA and ionomycin for 6 hours and analyzed by flow cytometry. Contour plots show gating strategy that was applied to patient samples in Fig. 4.