Extended Data Fig. 5: Gating strategy for the IMMUNOME tubes described in Supplementary Table 5.

(a) Tubes 1–11 were gated on the basis of CD45 and side scatter. The immune cell populations of interest were those falling within the region ‘lymphocytes,’ which was defined by CD45^bright, a low side scatter, and a lymphosum (CD3⁺ CD19⁺ CD56⁺ CD16⁺) totaling 95%–100%, with <5% myeloid contamination. Tube 1 (lymphosum) contained lymphosum markers, as well as myeloid markers (CD13/CD14). The gate established for Tube 1 was applied to Tubes 2–11. (b) Tube 12 (myeloid cells) used LIN (CD3⁺ CD19⁺ CD56⁺) as an exclusion gate. Plots were gated using three different strategies: LIN⁻ total, LIN⁻ CD11b⁺, and LIN⁻ CD33⁺. (c) Tube 13 (senescent cells) used both lymphocyte and sequential gating to find the immune cell population of interest. Lymphocyte gating isolated CD28⁻ CD16⁻ CD56⁻ CD3⁺ cells, which were sequentially gated on CD57. Events falling with the CD57⁺ region were considered senescent cells. Senescent cells were further classified into subsets defined by positivity and negativity for CD4, CD8, KLRG1, and CD127. (d) Tube 14 (dendritic cells) used LIN as an exclusion gate. Plots were gated using three different strategies: LIN⁻ total, LIN⁻ CD1c⁺, and LIN⁻ CD141⁺. CD, cluster of differentiation; KLRG1, killer cell lectin-like receptor subfamily G member 1; LIN, lineage.