Extended Data Fig. 5: Particulates inhibit phagocytosis but do not alter age-associated effects on proliferation.
a. Particulate containing THP-1 macrophages cannot phagocytose fluorescent pHrodo beads. Representative images of differentiated THP-1 cells previously incubated with (+) or without (-) black particulates subsequently cultured with pHrodo beads at 37 °C or 4 °C for 90 minutes. Confocal image of THP-1 cells imaged for pHrodo beads and brightfield. Yellow arrows indicate THP-1 cells. Scale bar: 50um. Image is representative of two experiments. (n = 9−12 wells/condition). b. Imaging data from Fig. 3g analyzed for Ki67+ CD68+CD169− macrophages that are within or outside the black particulate area. Significance assessed with paired T-test (two-tailed). Data are from 13 donors (2–7 single images for each donor).
